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Image Search Results
Journal: bioRxiv
Article Title: The Omicron variant BQ.1* with mutations at positions 28,311 and 28,312 in the SARS-CoV-2 N gene have minimal impact on CDC N1 target detection
doi: 10.1101/2023.01.26.525759
Figure Lengend Snippet: Amplification efficiency was evaluated in triplicate over a 7-log range (10 -10 copies/reaction) of synthetic wild-type RNA (black) versus the UU mutant RNA (green). Detection sensitivity was evaluated with 10 copies of RNA per reaction with 27 replicates per condition. NEB SARS-CoV-2 RT-qPCR Multiplex Assay Kit (NEB E3019) was used to detect A) N1 (HEX) and B) N2 (FAM) targets simultaneously following cycling conditions as recommended by the manufacture. All twenty-seven low copy replicates (10 copies) were detected successfully using CDC 2019-nCo_N1 primers and probe.
Article Snippet: The mutant and wild-type target RNA were subsequently amplified using either the NEB
Techniques: Amplification, Mutagenesis, Quantitative RT-PCR, Multiplex Assay